A rapid technique for the detection of wheat–rye translocation chromosomes

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Abstract
After 1–2 days on nitrocellulose filters kept moist with Tris–NaCl–EDTA (TNE) solution, sufficient DNA is adsorbed from individual half seeds of wheat to allow rye heterochromatin sequences to be detected in the adsorbed samples. Using the probe pSchet1, seeds of rye (Secale cereale), triticale (× Triticosecale), wheat (Triticum aestivum), and wheat containing 1RS/1BL translocation chromosomes have been successfully screened for the presence or absence of this heterochromatin sequence. The system has the potential to recognise the presence of the short arm of chromosome 1R of rye, and the presence of other rye DNA sequences, in thousands of samples per week.Key words: wheat, 1RS/1BL translocation, rye, heterochromatin, pSchet1

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