Adaptation of an Alkaline Phosphatase Method for Automatic Colorimetric Analysis
- 1 April 1959
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 5 (2) , 119-126
- https://doi.org/10.1093/clinchem/5.2.119
Abstract
The alkaline phosphatase method of Kind and King was adapted to an automated recording colorimeter. The precision of the automated method (1 standard deviation as per cent of the mean value) was ±1.7 and for the manual method ±3.6 per cent. The color produced was proportional to the enzyme concentration by both methods, and recoveries of added phenol were satisfactory. In more than 150 serum specimens surveyed for enzyme activity, over 95 per cent of the results (2 standard deviations) of the 2 methods in the range 3.4-129 agree to within ±2.8 King-Armstrong units/1OO ml.Keywords
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