On‐line capillary electrophoresis‐mass spectrometry for the analysis of biomolecules
- 14 July 2004
- journal article
- review article
- Published by Wiley in Electrophoresis
- Vol. 25 (14) , 2257-2281
- https://doi.org/10.1002/elps.200405954
Abstract
Mass spectrometry (MS) has become a key tool for the characterization of biologically relevant molecules in the last decade. Due to the complexity of most biological samples an upstream separation is essential. Capillary electrophoresis (CE) has gained much interest due to its high separation efficiency, speed, and often complementary selectivity to liquid chromatography. We describe the state‐of‐the‐art of on‐line CE‐MS for the analysis of molecules of biological origin. The characterization of peptides, including the study of post‐translational modifications, intact proteins, oligonucleotides, and related interaction studies are reviewed. Relevant publications are summarized in tables, including some important method parameters. Key applications are discussed with respect to the advantages and limitations of CE‐MS. Coupling interfaces, preconcentration techniques, capillary coatings, and the different CE techniques, e.g., capillary zone electrophoresis, capillary isoelectric focusing, capillary gel electrophoresis, etc. are briefly discussed against the background of their bioanalytical applications.Keywords
This publication has 100 references indexed in Scilit:
- Mass spectrometry for the detection of differentially expressed proteins: a comparison of surface‐enhanced laser desorption/ionization and capillary electrophoresis/mass spectrometryRapid Communications in Mass Spectrometry, 2003
- Applications of affinity interactions in capillary electrophoresisElectrophoresis, 2003
- Capillary electrophoresis coupled to time of flight‐mass spectrometry of therapeutic peptide hormonesElectrophoresis, 2003
- Identification of degradation products of aspartyl tripeptides by capillary electrophoresis-tandem mass spectrometryElectrophoresis, 2003
- Separation and identification of platinum adducts with DNA nucleotides by capillary zone electrophoresis and capillary zone electrophoresis coupled to mass spectrometryElectrophoresis, 2001
- An on-line preconcentrator and the evaluation of electrospray interfaces for the capillary electrophoresis/mass spectrometry of peptidesRapid Communications in Mass Spectrometry, 1999
- Pressure-assisted and pressure-programmed capillary electrophoresis/electrospray ionization time of flight - mass spectrometry for the analysis of peptide mixturesElectrophoresis, 1998
- Capillary zone electrophoresis of proteinsElectrophoresis, 1997
- Probing the microheterogeneity of O-specific chains fromYersinia ruckeri using capillary zone electrophoresis/electrospray mass spectrometryElectrophoresis, 1996
- Capillary isotachophoresis with UV and tandem mass spectrometric detection for peptides and proteinsElectrophoresis, 1990