High Performance Liquid Chromatographic Separation and Fluorescent Measurement of Taurine, a Key Amino Acid
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 2 (6) , 809-821
- https://doi.org/10.1080/01483917908060105
Abstract
A rapid, sensitive method for the analysis of taurine in oyster hemolymph (blood) has been developed. Highly fluorescent, substituted isoindoles formed by the reaction of taurine and other amino acids with o-phthaldialdehyde/ethanethiol reagent were separated on a reverse phase, high performance liquid chromatographic column. It was necessary to carefully control the concentration of the sodium ion in the phosphate buffer in order to maintain an adequate resolution of both taurine and tyrosine from β-alanine and arginine. Calibration plots of the fluorescent taurine derivative were linear over 2.5 orders of magnitude with a limit of detection of 0.10 nanomoles per ml of oyster hemolymph.This publication has 20 references indexed in Scilit:
- Comparison of ninhydrin, fluorescamine, and o-phthaldialdehyde for the detection of amino acids and peptides and their effects on the recovery and composition of peptides from thin-layer fingerprintsAnalytical Biochemistry, 1977
- Separation of amino acids by high performance liquid chromatographyAnalytical Chemistry, 1976
- Fluorescence reactions of aminophosphonic acidsAnalytical Chemistry, 1976
- O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin.Proceedings of the National Academy of Sciences, 1975
- Separations on heavily loaded small particle columns in high speed liquid chromatographyAnalytical Chemistry, 1974
- Novel reagent for the fluorometric assay of primary aminesJournal of the American Chemical Society, 1972
- Condensation of ninhydrin with aldehydes and primary amines to yield highly fluorescent ternary productsAnalytical Biochemistry, 1971
- Fluorescence reaction for amino acidsAnalytical Chemistry, 1971
- Hemolymph-free amino acids and related nitrogenous compounds of Crassostrea virginica infected with Bucephalus sp. and Minchinia nelsoniComparative Biochemistry and Physiology, 1970
- Ion Exchange Chromatography of Amino Acids. Semiautomatic Method of Operation with Cationic Exchange Resin ColumnsAnalytical Chemistry, 1959