Formation of 5,6-dihydroxydihydrothymine-type Products in DNA by Hydroxyl Radicals

Abstract
Bacteriophage T7-DNA and HeLa S-3 cells were used with 4 scavengers (methanol, glycerol, ethylene glycol and cysteine). The material was irradiated by a 137Cs source. The hydroxyl radical was primarily responsible for the formation of products of the 5,6-dihydroxydihydrothymine type when T7-DNA or intact HeLa cells were irradiated with X-rays.