THE PERMEABILITY OF THE HUMAN CILIARY AND IRIDIAL EPITHELIUM TO HORSERADISH PEROXIDASE

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Abstract
The permeability of the human ciliary epithelium to horseradish peroxidase (PO) was studied in vitro by EM. Ciliary body and iris specimens were obtained from freshly enucleated eyes. PO was applied at the stromal side of the epithelium, and was left for 120 min. The movement of PO through the intercellular spaces of the human ciliary epithelium was blocked apically in the lateral intercellular spaces of the non-pigmented epithelial cells, indicating that these cells are girdled by zonulae occludentes. In the iridial epithelium, the same distribution pattern of peroxidase reaction products (PORP) was found, i.e., the progression of PO was blocked apically in the lateral intercellular spaces of the posterior epithelial cells. Apparently the human ciliary and iridial epithelium contains a system of zonulae occludentes, which represents a diffusion barrier to high molecular, water soluble substances. This is consistent with previous studies in several species of animals.