The Large Scale Laboratory Culture of Chlorella Under Conditions of Micronutrient Element Deficiency

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Abstract
An apparatus has been devised whereby large quantities of Chlorella can be grown under conditions controlled with respect to minor element concentration. This equipment allows for rapid, intermittent sampling and for addition of materials without ensuing contamination. Under uniform conditions comparable growth rates have been attained among six replicate cultures of two liters each. Mn and Fe deficiencies have been deomonstrated, and a Mn requirement of 2.9 [mu]g per g dry weight has been determined for the species used (C. vulgaris var. viridis).