The crystal structures of reduced pseudoazurin from Alcaligenes faecalis S‐6 at two pH values
- 27 June 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 347 (2-3) , 203-206
- https://doi.org/10.1016/0014-5793(94)00544-3
Abstract
The structures of the reduced (Cu1+) blue-copper protein pseudoazurin from Alcaligenes faecalis strain S-6 are refined at pH 7.8 and 4.4 using X-ray diffraction data to 1.8 Å resolution. The final R-factors for the high and low pH structures are 0.178 and 0.177, respectively. Comparing the reduced pseudoazurin at pH 7.8 with the oxidised (Cu2+) molecule, small changes are observed in the vicinity of the copper site and on the protein surface. At pH 4.4 the copper substituent imidazole of His81 rotates away from the metal with a concurrent movement of the latter towards the plane of the remaining three ligands (Sγ-Cys78, Nδl-His40 and Sδ-Met86) thus the geometry of the copper site becomes planar trigonal.Keywords
This publication has 14 references indexed in Scilit:
- A 1H‐NMR study on the blue copper protein amicyanin from Thiobacillus versutusEuropean Journal of Biochemistry, 1988
- The blue copper protein gene of Alcaligenes faecalis S-6 directs secretion of blue copper protein from Escherichia coli cellsJournal of Bacteriology, 1987
- The crystal structure of pseudoazurin from Alcaligenes faecalis S‐6 determined at 2.9 Å resolutionFEBS Letters, 1987
- Crystal structure analyses of reduced (CuI) poplar plastocyanin at six pH valuesJournal of Molecular Biology, 1986
- Electron transfers in chemistry and biologyBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1985
- Tilden Lecture. Structure and electron-transfer reactivity of the blue copper protein plastocyaninChemical Society Reviews, 1985
- Purification and Properties of a Copper-Containing Nitrite Reductase from a Denitrifying Bacterium, Alcaligenes faecalis Strain S-6The Journal of Biochemistry, 1981
- A graphics model building and refinement system for macromoleculesJournal of Applied Crystallography, 1978
- X-ray crystal structure analysis of plastocyanin at 2.7 Å resolutionNature, 1978