Phosphorylation control by insulin in adipocytes is interfered with at a post‐receptor step by phosphoinositol and glucosamine
- 30 July 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 268 (1) , 169-172
- https://doi.org/10.1016/0014-5793(90)81000-e
Abstract
Inositol‐phosphates, glucosamine and glucose‐6‐phosphate blocked the effects of insulin on target protein phosphorylation in adipocytes, but the unsubstituted or sulphated derivatives of inositol or of glucose, or N‐acetyl‐glucosamine were without effect. The insulin stimulated tyrosine phosphorylation of the insulin receptor was not affected. The sugar‐phospates inositol‐phosphate and glucose‐6‐phosphate did not enter into the cells. They also blocked the insulin‐like effects of a potential second messenger of insulin, a phosphooligosaccharide (POS), which has previously been shown to mimick the effects of insulin on protein phosphorylation in intact cells.Keywords
This publication has 32 references indexed in Scilit:
- Rat liver insulin mediator which stimulates pyruvate dehydrogenase phosphatase contains galactosamine and D-chiroinositolPublished by Elsevier ,2005
- Asymmetric distribution of the phosphatidylinositol‐linked phospho‐oligosaccharide that mimics insulin action in the plasma membraneEuropean Journal of Biochemistry, 1990
- Regulation of protein phosphorylation by insulin and an insulinomimetic oligosaccharide In 3T3-L1 adipocytes and Fao hepatoma cellsBiochemical and Biophysical Research Communications, 1988
- Localisation of the insulin-sensitive phosphatidylinositol glycan at the outer surface of the cell membraneBiochemical and Biophysical Research Communications, 1988
- Phospho-dephospho-control by insulin is mimicked by a phospho-oligosaccharide in adipocytesNature, 1987
- A phospho-oligosaccharide mimics insulin action on glycogen phosphorylase and pyruvate kinase activities in isolated rat hepatocytesBiochemical and Biophysical Research Communications, 1987
- Partial structure of an insulin-sensitive glycophospholipidBiochemical and Biophysical Research Communications, 1987
- The polar head group of a novel insulin‐sensitive glycophospholipid mimics insulin action on phospholipid methyltransferaseFEBS Letters, 1986
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970