Observations on the Hemolytic Mechanism of Paroxysmal Nocturnal Hemoglobinuria

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Abstract
1. It has been shown that PNH hemolysis can be inhibited in vitro in the following ways: (a) addition of fluoride, phosphate, citrate, oxalate and calcium ions; (b) inactivation at 56 C.; (c) adsorption of a globulin from serum. 2. The action of at least two serum factors ins PNH hemolysis has been demonstrated. One of these factors is heat labile and magnesium dependent and the other is an adsorbable globulin. 3. It is believed that the heat labile factor must react with the cells prior to the adsorbable factor if hemolysis is to occur. 4. The resemblance of these factors to the components of complement is briefly discussed.