Development of a Recombinant Baculovirus Expressing an Insect-Selective Neurotoxin: Potential for Pest Control
- 1 September 1991
- journal article
- research article
- Published by Springer Nature in Nature Biotechnology
- Vol. 9 (9) , 848-852
- https://doi.org/10.1038/nbt0991-848
Abstract
Recombinant nuclear polyhedrosis viruses (NPVs) expressing insect-selective toxins, hormones, or enzymes could enhance their insecticidal properties. We have constructed a recombinant, polyhedrin-positive Autographa californica NPV (AcNPV) that is orally infectious and expresses an insect-selective toxin (AalT), isolated from the scorpion Androctonus australis, under the control of the p10 promoter. Bioassays with the recombinant baculovirus on 2nd instar larvae of Heliothis virescens demonstrated a significant decrease in the time to kill (LT50 88.0 hours) compared to wild-type AcNPV (LT50 125 hours). Production of AalT was confirmed by western blot analysis of larval hemolymph from infected H. virescens, and bioassays with larvae of Sarcophaga falculata.Keywords
This publication has 22 references indexed in Scilit:
- Insecticidal effects of an insect-specific neurotoxin expressed by a recombinant baculovirusVirology, 1991
- Analysis of very late gene expression by Autographa californica nuclear polyhedrosis virus and the further development of multiple expression vectorsJournal of General Virology, 1990
- Expression and effects of the juvenile hormone esterase in a baculovirus vectorNature, 1990
- Increased insecticidal effect by a recombinant baculovirus carrying a synthetic diuretic hormone geneBiochemical and Biophysical Research Communications, 1989
- Expression of Foreign Genes in Insects Using Baculovirus VectorsAnnual Review of Entomology, 1989
- Baculoviruses as Gene Expression VectorsAnnual Review of Microbiology, 1988
- The binding of the insect selective neurotoxin (AaIT) from scorpion venom to locust synaptosomal membranesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1984
- Selective binding of the scorpion venom insect toxin to insect nervous tissueInsect Biochemistry, 1979
- Action of different toxins from the scorpion Androctonus australis on a locust nerve-muscle preparationJournal of Insect Physiology, 1976
- The effect of scorpion venom on blowfly larvae—A new method for the evaluation of scorpion venoms potencyToxicon, 1971