Isopeptide linkage between nonhistone and histone 2A polypeptides of chromosomal conjugate-protein A24.
- 1 March 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (3) , 864-868
- https://doi.org/10.1073/pnas.74.3.864
Abstract
Chromosomal protein A24 [calf thymus] has a unique structure inasmuch as it contains histone 2A and a nonhistone polypeptide the sequence of which was partially determined. Comparative analysis of the ninhydrin-insensitive amino-terminal tryptic peptides of protein A24 and histone 2A and a quantitative analysis of their carboxyl-terminal amino acid indicated that protein A24 has 2 amino termini and 1 carboxyl terminus. The amino acid sequence analysis of tryptic peptide 17'' of protein A24 showed it contains tryptic peptide 17 of histone 2A, Lys-Thr-Glu-Ser-His-His-Lys. Lysine 119, the amino terminus of this peptide, which is derived from the histone 2A portion of protein A24, is linked by an isopeptide bond to the carboxyl group of a glycine residue. A branched structure of protein A24 is proposed.Keywords
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