Characterization of Mg2+ efflux from human, rat and chicken erythrocytes
- 3 July 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 250 (2) , 633-637
- https://doi.org/10.1016/0014-5793(89)80812-9
Abstract
Net Mg2+ efflux from Mg2+‐loaded human, rat and chicken erythrocytes was measured in sucrose, NaCl and choline Cl medium. Thus, Na+‐dependent (NaCl minus choline Cl) and Na+‐independent Mg2+ efflux (in sucrose) were determined. Na+‐dependent Mg2+ efflux amounted to 0.16, 8.9 and 1.57 mmol/l cells × 30 min, Na+‐independent Mg2+ efflux amounted to 0.89, 1.55 and 0.37 mmol/l cells × 30 min for human, rat and chicken erythrocytes. Na+‐dependent Mg2+ efflux was inhibited by quinidine. Na+‐independent Mg2+ efflux was inhibited by SITS and Cl−. A small fraction of Na+‐independent Mg2+ efflux (in choline Cl) was resistant to SITS and Cl−. Ca2+ loading increased Mg2+ efflux similar to K+ efflux (Gardos effect). This effect was differently expressed in human and chicken erythrocytes.Keywords
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