Testosterone concentrations in human seminal plasma and saliva and its correlation with non‐protein‐bound and total testosterone levels in serum

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Abstract
A sensitive, specific and precise non-chromatographic method for the radioimmunoassay of testosterone in human seminal plasma and saliva from adult and pubertal males is described, and the values compared to total and non-protein-bound testosterone levels in serum. There was a significant correlation between salivary and serum-free levels of testosterone (r = 0.75, P < 0.001, n = 67) while the correlation of total testosterone with free as well as with salivary testosterone levels was weaker (r = 0.63 and 0.64, respectively). The salivary and serum levels of free testosterone showed better correlation with the stage of puberty than did the serum levels of total testosterone. Further evidence for a correlation between salivary and serum levels of free testosterone was obtained following oral administration of testosterone undecanoate, as this treatment increased the mean concentration of serum total testosterone after 3 h by 82%, but increased salivary and serum levels of free testosterone by only 30% and 20%, respectively. The coefficient of correlation between serum levels of total testosterone and seminal plasma testosterone was 0.73 (P < 0.001), while the correlation between levels of serum-free testosterone with both salivary and seminal plasma levels of testosterone was statistically non-significant. Observation on salivary testosterone are in accordance with the diffusion of non-protein-bound steroids into peripheral tissues and consequently into their secretions. This model, however, does not appear to be applicable to the sex accessory glands.