Development of a Solid Phase Enzyme Immunoassay for the Detection of Anti-Ro Autoantibodies
- 1 January 1991
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Rheumatology
- Vol. 20 (4) , 267-273
- https://doi.org/10.3109/03009749109096799
Abstract
An enzyme immunoassay was developed to detect anti-Ro(SS-A) autoantibodies. Both Ro-antigen components (52 and 60 kD) were purified from a pig spleen extract, using fast protein liquid chromatography (FPLC). Anti-La, anti-RNP, anti-DNA and anti-Sm antibodies do not react to the purified antigen. There was a strong correlation between anti-Ro activity in EIA and the titers in counter Immunoelectrophoresis (rs= 0,893). Anti-Ro antibodies were found in 54 (69.2%) of 78 SLE sera by the developed EIA.Keywords
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