Investigations on myelination in vitro: Thyroid hormone receptors in cultures of cells dissociated from embryonic mouse brain

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Abstract
Brain cells dissociated from 15 day old embryonic mice and grown in culture contain cytosolic and nuclear receptors for L-3,5,3''-triiodothyronine (L-T3). Kd values for L-T3 of 3.05 .times. 10-9 and 4.2 .times. 10-9 M were determined with the cytosolic and nuclear receptors, respectively. These cultured cells, which are suitable for studying the regulation of myelination by T3 in vitro, display a high specificity for L-T3 in that the receptors for L-T3 do not bind D-T3, D-thyroxine, L-diiodothyronine or DL-thyronine and bind only small amounts of L-thyroxine.