Interspecific variations in proteins synthesized by mammalian mitochondria

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Abstract
The products of mitochondrial protein synthesis in established cell lines of various mammalian species [Chinese hamster CHOTK-, bovine EBTR, mouse L, rat HTC, human WI-38, VA-13A (SV-40-transformed WI-38) and Syrian hamster BHK-21] were labeled with [35S]methionine and their number and apparent MW determined by sodium dodecyl sulfate polyacrylamide slab gel electrophoresis and fluorography. Proteins synthesized by isolated rat liver mitochondria were labeled with [3H]valine and similarly characterized. Each species had a distinctive pattern of from 10-13 mitochondrially synthesized proteins with apparent MW between 10,000-50,000. No differences were detected in the number or electrophoretic mobility of the mitochondrially synthesized proteins of SV-40-transformed and nontransformed WI-38 cells.