Blood-Surface interactions: Fourier transform infrared studies of protein surface adsorption from flowing blood plasma and serum

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Abstract
The adsorption of blood proteins onto various surfaces in contact with flowing blood plasma or serum has been investigated using Fourier Transform infrared spectroscopy (FT-IR) coupled with attenuated total reflection (ATR). Comparison of adsorption from a static versus a flowing blood plasma system indicated that a greater amount of protein was adsorbed when the static system was employed, but desorption was observed with the static system and not in the flowing system. When plasma and serum were compared in terms of amount and type of protein adsorbed, little difference was noted in either kinetics of adsorption or stability of the adsorbed layers. Variations in the ratios of infrared adsorption bands (from either plasma or serum) indicate that changes occur during the adsorption process in the protein layers. A comparison of poly(vinyl chloride) to germanium as the protein adsorbing surface in the static system showed differences in terms of the composition of the adsorbed layers.