Separation of Cells Exhibiting Acid Phosphatase Activity From Disaggregated Hamster Prostate Cells in an Isokinetic Gradient of Ficoll in Tissue Culture Medium 2
- 1 January 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 54 (1) , 147-150
- https://doi.org/10.1093/jnci/54.1.147
Abstract
Cells exhibiting histochemically demonstrable acid phosphatase were separated from suspensions of hamster prostate cells with velocity and isopyknic sedimentation. Unseparated suspensions of hamster prostate cells contained 57.l ± 11.3% cells with histochemically apparent acid phosphatase. After the cells were separated by velocity sedimentation in a previously described isokinetic gradient, the purest fractions from the gradient contained 97.2 ± 0.8% cells with histochemically evident acid phosphatase. More than 99% of these separated cells excluded trypan blue. These cells were thought to be the acinar cells of the prostate. Isopyknic sedimentation was not as effective as velocity sedimentation for the purification of these cells.Keywords
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