Sequence-independent amplification and labeling of yeast artificial chromosomes for fluorescence in situ hybridization
- 1 January 1994
- journal article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 65 (1-2) , 108-110
- https://doi.org/10.1159/000133612
Abstract
We have developed a method that allows reliable construction of high quality FISH probes from yeast artificial chromosomes (YACs) based on the separation of YACs by pulsed-field gel electrophoresis and a rapid sequence-independent amplification procedure (SIA). These probes can be used to localize YACs on metaphase chromosomes and also with high efficiency, in interphase nuclei.Keywords
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