Cell wall glycoproteins from Chlamydomonas reinhardii, and their self-assembly
- 1 January 1978
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 138 (1) , 91-98
- https://doi.org/10.1007/bf00392922
Abstract
We have investigated the in vitro reassembly of the salt soluble, hydroxyproline rich, glycoproteins from the cell wall of Chlamydomonas reinhardii, into structured cell wall fragments. We have devised an assay which has been used to follow the reassembly of the unfractionated and fractionated (2BI and 2BII) cell wall glycoproteins. Reassembly has a pH optimum of 5, a temperature optimum of 20°C, and the final size of the reassembled fragments appears to be promoted by the minor component 2BI. Periodate oxidation experiments show that sugar residues, in particular mannose, are important for accurate reassembly. Using electron microscopy, the structure of the reassembled products has been elucidated, as have intermediate stages in the reassembly process.Keywords
This publication has 16 references indexed in Scilit:
- A structural glycoprotein, containing hydroxyproline, isolated from the cell wall of Chlamydomonas reinhardiiPlanta, 1976
- Some properties of carbohydrate-binding proteins (lectins) solubilized from cell walls of Phaseolus aureusPlanta, 1976
- Self-assembly of a plant cell wall in vitroJournal of Molecular Biology, 1975
- Turbidimetric studies of the in vitro assembly and disassembly of porcine neurotubulesJournal of Molecular Biology, 1974
- Crystalline glycoprotein cell walls of algae: their stucture, composition and assemblyPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1974
- Maturation of the head of bacteriophage T4Journal of Molecular Biology, 1973
- Structure, composition, and morphogenesis of the cell wall of Chiamydomonas reinhardi: II. Electron microscopy and optical diffraction analysisJournal of Ultrastructure Research, 1973
- Structure, composition and morphogenesis of the cell wall of Chlamydomonas reinhardi: I. Ultrastructure and preliminary chemical analysisJournal of Ultrastructure Research, 1972
- Tertiary and quaternary structure in aqueous polysaccharide systems which model cell wall cohesion: Reversible changes in conformation and association of agarose, carrageenan and galactomannansJournal of Molecular Biology, 1972
- Measurement and compensation of defocusing and aberrations by Fourier processing of electron micrographsPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1971