Synthesis of Modified Oligonucleotides and Production of Duplexes With Covalently Linked Chains
- 1 April 1996
- journal article
- research article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 15 (4) , 923-936
- https://doi.org/10.1080/07328319608002138
Abstract
A method has been devised to synthesize a DNA-duplex with covalently connected strands. Primary amino group located on one strand is linked to a carboxyl group of the other strand through the agency of a water soluble carbodiimide condensing agent. Conditions for the reaction between chains of a duplex composed of the modified oligonucleotides [1] were optimized. The thermal and hydrolytic stability of the cross linked duplex was examined.Keywords
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