Molecular cloning and expression in Escherichia coli K-12 of chromosomal genes determining the O antigen of an E. coli O2: K1 strain
- 15 August 1991
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 82 (3) , 345-351
- https://doi.org/10.1016/0378-1097(91)90286-j
Abstract
The rfb gene cluster which determines the biosynthesis of the O2 O antigen has been cloned from an Escherichia coli O2: K1 strain isolated from a case of septicaemia in chickens. The region required for expression of the O antigen in E. coli K-12 was localised to a 10.7 to 14.15-kb segment which was shown to be chromosomal in origin with a close linkage to the gnd and his genetic loci.Keywords
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