THIOCHOLINE METHODS FOR THE DEMONSTRATION OF ACETYLCHOLINESTERASE IN NEUROMUSCULAR-JUNCTIONS

  • 1 January 1981
    • journal article
    • research article
    • Vol. 30  (118) , 69-82
Abstract
The extensor digitorum longus muscles of rat were stained for the localization of acetylcholinesterase activity at the neuromuscular junctions. The modified methods of Koelle-Friedenwald and Karnovsky-Roots were used with acetylthiocholine iodide as the substrate. The merits and demerits of both methods are discussed. TEM [transmission electron microscopy] and SEM [scanning electron microscopy] X-ray energy dispersive analyses of the muscle fibers treated histochemically by both methods were made to elucidate further the nature of the reaction products. Denervated muscles were subjected to similar treatment.