Concanavalin A-induced Nuclear Division in Stylonychia mytilus

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Abstract
The nuclear behavior of S. mytilus altered after treatment with Con A [concanavalin A]. Divisions of micro- and macronuclei led to abnormal numbers of nuclei after 5-6 h in all cells. In some cells up to 7 micronuclei and up to 3 macronuclei, often atypically round, could be seen at this time. Nuclear events were not accompanied by cell division which started only after shedding the Con-A loaded pellide. Autoradiography showed thymidine incorporation in many micronuclei within 6 h of Con A treatment, indicating that DNA synthesis was a consequence of lectin treatment. Thymidine incorporation could be quantitated by pulse-labeling. Macronuclei did not incorporate thymidine during the first 6 h after Con A treatment, which suggests fragmentation of these nuclei without DNA synthesis.

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