Regulation by glucocorticoids of angiotensinogen gene expression and secretion in adipose cells
Open Access
- 1 December 1997
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 328 (2) , 701-706
- https://doi.org/10.1042/bj3280701
Abstract
Adipose cells are an important source of angiotensinogen (AT). Its activation product, angiotensin II, stimulates in vitro and in vivo the production and release of prostacyclin which acts as a potent adipogenic signal in promoting the terminal differentiation of preadipocytes to adipocytes. Since glucocorticoids are known to promote adipose cell differentiation in vitro as well as in vivo, their role in the regulation of AT gene expression and secretion has been investigated in cultured Ob1771 mouse adipose cells. In contrast with liver cells, which are the major source of AT and the target of several hormones for the regulation of its expression, adipose cells are only responsive to glucocorticoids, which are able to up-regulate AT gene expression and AT secretion rapidly and dose-dependently. On exposure to glucocorticoids, accumulation of AT mRNA appears primarily to be due to transcriptional activation of the gene and is parallelled by secretion of the protein. Similar results on AT mRNA expression and AT secretion were obtained using explants of rat adipose tissue ex vivo demonstrating a major if not exclusive mechanism of regulation of AT production by glucocorticoids in mature adipose cells. Together these results provide a potential link between glucocorticoids, AT, the growth of adipose tissue and increased blood pressure.Keywords
This publication has 45 references indexed in Scilit:
- Evidence for a common mechanism of action for fatty acids and thiazolidinedione antidiabetic agents on gene expression in preadipose cells.1994
- Differentiation of preadipose cells: paracrine role of prostacyclin upon stimulation of adipose cells by angiotensin-II.Endocrinology, 1994
- The effects of cortisol on the regulation of lipoprotein lipase activity in human adipose tissue.Journal of Clinical Endocrinology & Metabolism, 1994
- Insulin-like growth factor-I is an essential regulator of the differentiation of 3T3-L1 adipocytes.Journal of Biological Chemistry, 1988
- Location and regulation of rat angiotensinogen messenger RNA.Hypertension, 1988
- Localization of angiotensinogen messenger RNA in rat aorta.Circulation Research, 1988
- Cellular Localization of Angiotensinogen Gene Expression in Brown Adipose Tissue and Mesentery: Quantification of Messenger Ribonucleic Acid Abundance Using Hybridization in Situ*Endocrinology, 1987
- Multiple Hormones Regulate Angiotensinogen Messenger Ribonucleic Acid Levels in a Rat Hepatoma Cell LineEndocrinology, 1987
- Circulating and tissue angiotensin systems.Journal of Clinical Investigation, 1987
- The Site of Angiotensin ProductionJournal Of Hypertension, 1985