Comparison of the Directigen Flu A+B Membrane Enzyme Immunoassay with Viral Culture for Rapid Detection of Influenza A and B Viruses in Respiratory Specimens
Open Access
- 1 August 2004
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 42 (8) , 3707-3710
- https://doi.org/10.1128/jcm.42.8.3707-3710.2004
Abstract
The performance of a commercially available, rapid membrane enzyme immunoassay for influenza A and B virus detection was compared to that of viral culture in 4,092 respiratory specimens collected from patients presenting with respiratory symptoms during the 2002-2003 influenza season. The test's overall sensitivity was 43.83%, lower than previously reported but similar for detection of both influenza A and B viruses (42.98 versus 44.76%). However, specificity, 99.74%, was excellent for both influenza A and B viruses (99.82 versus 99.92%). These values make this test a very good confirmatory test when clinical suspicion is high, but a less accurate screening test for large populations.Keywords
This publication has 17 references indexed in Scilit:
- Influenza vaccine enlisted to prevent SARS confusionThe Lancet, 2003
- Impact of the Rapid Diagnosis of Influenza on Physician Decision-Making and Patient Management in the Pediatric Emergency Department: Results of a Randomized, Prospective, Controlled TrialPediatrics, 2003
- Suboptimal Detection of Influenza Virus in Adults by the Directigen Flu A+B Enzyme Immunoassay and Correlation of Results with the Number of Antigen-Positive Cells Detected by Cytospin ImmunofluorescenceJournal of Clinical Microbiology, 2003
- SARS exposed, pandemic influenza lurksThe Lancet, 2003
- Comparison of Lateral-Flow Immunoassay and Enzyme Immunoassay with Viral Culture for Rapid Detection of Influenza Virus in Nasal Wash Specimens from ChildrenJournal of Clinical Microbiology, 2003
- Rapid diagnostic testing for the identification of respiratory agents in the emergency departmentClinical Pediatric Emergency Medicine, 2002
- Effectiveness of Reverse Transcription-PCR, Virus Isolation, and Enzyme-Linked Immunosorbent Assay for Diagnosis of Influenza A Virus Infection in Different Age GroupsJournal of Clinical Microbiology, 2002
- Evaluation of the Directigen FluA+B Test for Rapid Diagnosis of Influenza Virus Type A and B InfectionsJournal of Clinical Microbiology, 2002
- Laboratory diagnosis of influenza: Recent advancesSeminars in Pediatric Infectious Diseases, 2002
- Fatal influenza B virus pneumonia in pediatric patientsThe Pediatric Infectious Disease Journal, 1992