Mating Type Specific Inhibition of Gametic Differentiation of Chlamydomonas reinhardtii by Tunicamycin
- 1 December 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 22 (8) , 1607-1611
- https://doi.org/10.1093/oxfordjournals.pcp.a076314
Abstract
The effect of tunicamycin, an inhibitor of glycosylation of proteins, on the gametic differentiation of Chlamydomonas reinhardtii was studied. When mt+ cells were treated with tunicamycin during gametogenesis, the acquisition of agglutinability on their flagella was completely inhibited. However, no significant inhibition was observed when mt− cells were treated with tunicamycin during gametic induction. The agglutinability of the fully competent gametes of mt+ cells decreased sharply after about 4 hr of incubation with tunicamycin and was lost completely after 8 hr. These results indicate that the gametic flagellar membrane of the mt+ cell may acquire glycoproteins with tunicamycin sensitive sugar chains, the halflife of which is about 6 hr.This publication has 1 reference indexed in Scilit:
- Biosynthesis of N-glycosidically linked glycoproteins during gastrulation of sea urchin embryos.Published by Elsevier ,2021