Preparation and Properties of Sweet Potato Mitochondria

Publisher Website
Google Scholar
Abstract
Procedures are described for preparing mitochondria from tuber tissues of both sweet potato and white potato. Mitochondria prepared according to these procedures are tightly coupled and possess respiratory control. Sweet potato mitochondria oxidizing malate have much greater respiratory control then when oxidizing succinate. The oxidation of succinate by freshly prepared sweet potato mitochondria is inhibited and ATP is required to overcome this inhibition. The inhibition is caused by formation of an oxaloacetate-succinic dehydro-genase complex; there is an energy requirement for the dissociation of this complex. The effects of a variety of respiratory chain inhibitors are thoroughly investigated and reported.