Design of a Highly Efficient Extraction Column System Using an Aqueous Liquid-Liquid Distribution to Clean Up Biological Fluids

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Abstract
An improved column fractionation procedure was developed for the analysis of biological samples. According to the simulation of the solute distribution in 2 phases for various [drug] extraction models, a multi-step liquid-liquid partition technique employing packed columns was more effective than the usual funnel separation-batch method and the droplet current distribution procedure. As an alternative to the open-bed extraction column used for the pre-treatment of the biological fluids, a closed-bed column system was introduced through the incorporation of a UV monitor. Drugs and their metabolites in urine were extracted quantitatively using diethyl ether as a carrier solvent. The aqueous liquid-liquid distribution column having a high theoretical plate number was prepared using diatomaceous earth consisting of small size particles and applying a slurry packing procedure.

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