The quantitative extraction of carotene from grass

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Abstract
The majority of methods for determining carotene involve a preliminary determination of total carotenoids, followed by a phase separation of the two pigments. This phase separation considerably limits the usefulness of such methods, and in order to dispense with it a direct extraction of carotene is necessary. For this purpose petroleum ether may be used in the presence of alcohol.The use of hot alcoholic potash for the disintegration of leaf material and separation of chlorophyll leads to a very undesirable precipitate which hinders subsequent extraction. Two alternative methods are available, which overcome this difficulty, viz. (1) extraction with hot alcohol, followed by treatment of the extract with potash, and (2) saponification with hot aqueous potash, followed by filtration and extraction of the grass residue with alcohol. The second of these methods has been adopted, being of greater general utility and capable of producing complete cellular breakdown, which is essential for efficient extraction. Details of this extraction process have been described.A comparison of the results obtained, with those from two other methods, has been made by a colorimetric determination of the extracted pigment. The variance and standard error have been shown to be smaller than those for the other two methods, and it has been concluded that the results obtained are reproducible with considerable accuracy.

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