Paper Chromatographic Study of Diffusible Auxin

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Abstract
The diffusible auxin and indoleacetic acid (IAA) were examined by paper chromatography with a neutral solvent system [isopropanol: water (10:1 v/v)] in an acidic atmosphere to prevent effects of ammonia vapor. The identification of diffusible auxin as related to IAA was checked in each instance by adding synthetic IAA to the diffusate. Under these conditions the Rf of IAA was either 0.7-0.8 or 0.8-0.9. The Rf of diffusible auxin from coleoptile tips of Avena sativa variety Victory grown in the dark room was 0.6-0.7. The Rf of diffusible auxin from stem apices of Pisum sativum variety Alaska grown in the greenhouse was 0.5-0.6. The Rf of diffusible auxin from stem apices of Centaurea cyanus variety Blue Boy under short-day conditions was 0.5-0.8, while the Rf of diffusible auxin of these plants under long-day conditions was 0.4-0.5. In each instance the diffusible auxin was different from IAA. Auxin diffused from sunflower apices (Helianthus annuus variety Russion Mammoth) grown under sunlight (light-type auxin) had an Rf of 0.1-0.2 and that from sunflower apices kept in the dark (dark-type auxin) had an Rf of 0.7-0.8. The light-type auxin diffused from 8534 sunflower apices and separated chromatographically was Ehrlich positive indicating that it might be an indole compound.
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