Enzyme-coupled ultraviolet determination of alpha-amylase activity with the GEMSAEC centrifugal analyzer.
Open Access
- 1 September 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 24 (9) , 1620-1624
- https://doi.org/10.1093/clinchem/24.9.1620
Abstract
We evaluated the Harleco alpha-glucosidase/hexokinase/glucose-6-phosphate dehydrogenase-coupled alpha-amylase method, bu use of the GEMSAEC centrifugal analyzer. Performance evaluation included kinetic studies of substrate and maltose hydrolysis as well as effects of endogenous glucose and fructose. The reagent was found to give a linear response with alpha-amylase activity to greater than 1200 U/liter. Within-run precision resulted in coefficients of variation (CV) of 0.9 to 3.2% over the range studied. Day-to-day precision corresponded to CV's of 2.4 to 4.4% over the same range of alpha-amylase procedure was found to be good (r = 0.997) for patients' sera examined.This publication has 1 reference indexed in Scilit:
- Evaluation of the Du Pont aca alpha-amylase procedure.Clinical Chemistry, 1977