Fertility of Bovine Spermatozoa in Buffered Whole Egg Extenders Containing Penicillin, Streptomycin, Sulfonamides and Added Glucose

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Abstract
Three experiments were conducted, using the split sample technic, for comparing the fertility of bovine semen extended in 2.9% citrate-sulfanilamide-yolk, 2.9% citrate-succinylsulfathiazole-whole egg, and 2.3% citrate-succinylsulfanil-amide-whole egg-glucose. Each extender contained 500 units each of penicillin and streptomycin. The avg. 60- to 90-day % non-returns to a total of approx. 5,000 1st services/treatment was 71.5 for the yolk extenders containing antibiotics and 69.4 for the whole egg extenders containing antibiotics. From these results and from those reported previously, it appears that the standard 2.9% citrate-sulfanilamide-yolk extender is about 2 percentage units superior to the whole egg extenders when measured by the 60- to 90-day % non-returns to 1st services. Penicillin and streptomycin used together have similar effects on fertility, whether they are added to either the yolk or the whole egg formula.