Caged RNA: photo-control of a ribozyme reaction
- 1 July 1998
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 26 (13) , 3173-3178
- https://doi.org/10.1093/nar/26.13.3173
Abstract
We report here the first photo-chemical control of a ribozyme reaction by the site-specific modification of the 2'-hydroxyl nucleophile in the hammerhead system with a caging functionality. Rapid laser photolysis of the O-(2-nitrobenzyl) caging group initiates an efficient and accurate hammerhead-catalyzed cleavage of substrate RNA under native conditions. RNAs in which reactive functionalities or recognition elements are caged in this manner will be useful tools to probe RNA reactivity and dynamics.Keywords
This publication has 25 references indexed in Scilit:
- The Structural Basis of Hammerhead Ribozyme Self-CleavageCell, 1998
- Capturing the Structure of a Catalytic RNA Intermediate: The Hammerhead RibozymeScience, 1996
- Synthesis, Kinetics, and Structural Studies of a Photolabile Caged Isocitrate: A Catalytic Trigger for Isocitrate DehydrogenaseBiochemistry, 1996
- Synthesis and biochemical evaluation of RNA containing an intrahelical disulfide crosslinkChemistry & Biology, 1995
- RNA foldingCurrent Opinion in Structural Biology, 1995
- Kinetic Intermediates in RNA FoldingScience, 1994
- Branch nucleophile selection in pre-mRNA splicing: evidence for the bulged duplex model.Genes & Development, 1994
- Controlling Cell Chemistry with Caged CompoundsAnnual Review of Physiology, 1993
- Site-Specific Modification of Pre-mRNA: the 2′-Hydroxyl Groups at the Splice SitesScience, 1992
- Time-resolved X-ray crystallographic study of the conformational change in Ha-Ras p21 protein on GTP hydrolysisNature, 1990