Derivation of human embryonic stem cells from single blastomeres
- 9 August 2007
- journal article
- Published by Springer Nature in Nature Protocols
- Vol. 2 (8) , 1963-1972
- https://doi.org/10.1038/nprot.2007.274
Abstract
This protocol details a method to derive human embryonic stem (hES) cells from single blastomeres. Blastomeres are removed from morula (eight-cell)-stage embryos and cultured until they form multicell aggregates. These blastomere-derived cell aggregates are plated into microdrops seeded with mitotically inactivated feeder cells, and then connected with neighboring microdrops seeded with green fluorescent protein-positive hES cells. The resulting blastomere-derived outgrowths are cultured in the same manner as blastocyst-derived hES cells. The whole process takes about 3-4 months.Keywords
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