Transport of 2-deoxy-D-glucose by isolated protoplasts of the yeast, Kluyveromyces lactis
- 1 July 1982
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 28 (7) , 901-906
- https://doi.org/10.1139/m82-135
Abstract
Protoplasts of succinate- and glucose-grown Kluyveromyces lactis were generated by incubating cells with β-mercaptoethanol and β-glucuronidase in the presence of MgSO4 as osmotic stabilizer. Transport of 2-deoxyglucose by the protoplasts was comparable with that of the walled cells. Only protoplasts of succinate-grown cells exhibited a lag in glucose utilization. Removal of the wall by this technique does not result in loss of the inducible component of the glucose carrier via degradation or release.This publication has 4 references indexed in Scilit:
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- Production of protoplasts in different yeasts by MutanaseCanadian Journal of Microbiology, 1981
- Evidence for an inducible glucose transport system in Kluyveromyces lactisBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
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