Hydrophobic labeling of (sodium, potassium)-ATPase: further evidence that the .beta. subunit is embedded in the membrane bilayer
- 26 April 1983
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 22 (9) , 2296-2300
- https://doi.org/10.1021/bi00278a037
Abstract
O-Hexanoyl-3,5-diido-N-(4-azido-2-nitrophenyl)tyramine was used after photochemical conversion into the reactive nitrene to label (Na+,K+)-ATPase from Bufo marinus toad kidney. Immunochemical evidence indicates that the reagent labels both subunits of the enzyme in partially purified form as well as in microsomal membranes. These results support the view that the glycoprotein subunit, like the catalytic subunit, possesses hydrophobic domains by which it is integrated into the plasma membrane.This publication has 7 references indexed in Scilit:
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