An Ozone-Generating System and Chamber for Testing Injury to Cultured Cells

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Abstract
A system was constructed which alternately exposed cultured cells to specific concentrations of O3 in the gas phase and then to cell culture medium. It was designed to produce a constant mass transfer between the gas phase and the exposed cell phase. Monolayers of neonatal rat lung fibroblasts cultured in miniature glass dishes were used to test for O3 toxicity. The cells were alternately exposed to O3 (0.05, 0.15, 0.45, 1.35 and 4.05 ppm) for 30 s and then to culture medium for 30 s over a 1 h period. Toxicity was measured as inhibition of cell growth 4 days after O3 exposure. Growth was significantly inhibited at all concentrations with a 50% inhibitory concentration of 0.8 ppm. The system described was apparently effective for quantifying O3 toxicity for cultured lung cells.

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