Connectin causes aggregation of myosin rods but not of myosin heads

Abstract
Connectin filaments linking myosin filaments to Z lines in chicken skeletal muscle interact with myosin rods but not with S1 heads in vitro. An increase in turbidity of myosin rods, and heavy and light meromyosins was observed in the presence of connectin in KCl concentrations lower than 0.15 M, similarly to the case of intact myosin. The aggregate formation of myosin rods in the presence of connectin was observed by electron microscopy. It appears that the S2 portion of myosin does not bind to connectin.