Evaluation of Protocol Using Gene Capture and PCR for Detection of Helicobacter pylori DNA in Feces

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Abstract
The route of transmission of Helicobacter pylori , which is usually acquired in childhood and is one of the most common bacterial infections in humans, remains undetermined. Mapping the distribution of H . pylori genotypes within families could help to determine the routes of transmission and risk factors. Here we describe a noninvasive method for obtaining H . pylori DNA isolates from the feces of children. Children presenting with gastrointestinal symptoms at the Royal Hospital for Sick Children were tested for gastric H . pylori colonization by using the 13 C-urea breath test (UBT) and were asked to provide fecal samples, which were tested for H . pylori by using the HpSA fecal antigen test. DNA was purified from fecal samples by using a novel method of gene capture with subsequent H . pylori PCR analysis. Fifteen UBT-positive and 15 UBT-negative children participated in the study. The positive and negative predictive values for the assay were 80 and 100%, respectively. Fecal DNA purification followed by H . pylori PCR analysis is an effective tool for harvesting H . pylori DNA isolates from the feces of children. This technique may be developed to allow the diagnosis and noninvasive genotyping of H . pylori in children and their families.