FIELD-EVALUATION OF ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR DETECTION OF ANTIBODY TO AFRICAN SWINE FEVER VIRUS

  • 1 January 1981
    • journal article
    • research article
    • Vol. 42  (8) , 1441-1443
Abstract
The enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to African swine fever [ASF] virus was evaluated under field conditions in the Dominican Republic. A total of 3402 swine sera were tested by ELISA. All 224 ELISA-positive sera and 426 of 2760 ELISA-negative sera were retested by indirect immunofluorescence (IIF). There were no antibodies to ASF virus detected by IIF in ELISA-negative sera. By IIF, antibodies were detected in only a small portion of the ELISA-positive sera. The specificity and sensitivity of ELISA and IIF were investigated and discussed. Poor quality sera had an effect in ELISA results. Detection of IgG and IgM antibodies in the ELISA and IIF-positive sera were also performed and related to longevity of ASF virus infection. The ELISA is recommended as an efficient method in testing large numbers of sera; positive results are to be retested by IIF.

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