Distribution of zinc amongst human serum globulins determined by gel filtration-affinity chromatography and atomic-absorption spectrophotometry
- 1 January 1984
- journal article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 109 (6) , 709-711
- https://doi.org/10.1039/an9840900709
Abstract
Gel-filtration chromatography was combined with affinity chromatography (for albumin) and electrothermal atomic-absorption spectrophotometry to investigate the distribution of zinc amongst normal human serum globulins. Most of the zinc associated with serum globulins appeared to be bound to α2-macroglobulin, and the observed zinc content of α2-macroglobulin was in very good agreement with that found using more complex techniques. No zinc was found in association with transferrin and the recovery of globulin-bound zinc was quantitative, 104%.Keywords
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