Clear distinction of pyrimidine bases on the complementary strand by fluorescence change of novel fluorescent nucleosides
Open Access
- 1 September 2003
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Symposium Series
- Vol. 3 (1) , 171-172
- https://doi.org/10.1093/nass/3.1.171
Abstract
New fluorescent nucleosides, methoxybenzodeazaadenine (MDA) and methoxybenzodeazainosine (MDI) which can sharply distinguish between C and T bases, respectably. The hybridization of an ODN probe containing MDA and MDI with a target DNA facilitates the judgment with the fluorescence spectra of the type of pyrimidine bases located at a specific site on the target DNA. The MDA- and MDI containing ODN are very effective probes for pyrimidine SNP typing.Keywords
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