Temperature-sensitive mutants of the mycobacterium plasmid pAL5000
- 1 November 1992
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 98 (1-3) , 181-186
- https://doi.org/10.1016/0378-1097(92)90152-e
Abstract
Two plasmids were isolated as thermosensitive replicons following in vitro mutagenesis of pB4, a pAL5000 derivative mycobacteria/Escherichia coli shuttle plasmid. Plasmids pCG59 and pCG63 replicate at 30°C but not at 39°C. This will allow their utilisation for transposon delivery, site-specific integration, or allele exchange.Keywords
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