Stimulation of mono (ADP‐ribosyl)ation by reduced extracellular calcium levels in human fibroblasts
- 1 January 1988
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 134 (1) , 161-165
- https://doi.org/10.1002/jcp.1041340121
Abstract
Lowering extracellular calcium in cultures of human diploid fibroblast-like cells caused a rapid depletion of NAD pools. This loss of NAD was reversed by restoring extracellular Ca2+ and was inhibited by 3-aminobenzamide, an inhibitor of ADP-ribosyl transfer reactions. The concentrations of 3-aminobenzamide needed to inhibit the loss of NAD were consistent with those required to inhibit cellular mono(ADP-ribosyl) rather than poly(ADP-ribosyl) reactions. Calcium depletion did not inhibit the biosynthesis of NAD. These results suggest that mono(ADP-ribosyl)ation is involved in the regulation of cellular Ca2+ levels.This publication has 28 references indexed in Scilit:
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