Inhibition of the Nitrate Reductase Complex by Dibromothymoquinone

Abstract
The plastoauinone antagonist 2,5-dibromothymoquinone was found to inhibit NO3 - reduction from NADH by the nitrate reductase complex from wheat. It accepts electrons from NADH through the NADH dehydrogenase activity of the nitrate reductase. However, it does not inhibit the reduction of 2,6-dichlorophenol-indophenol by the enzyme. This suggests that the two com­pounds may be accepting electrons at different places from the enzyme. Further it was observed that reduced DCIP could be oxidized by DBMIB in the absence of NADH indicating that the electron flow in the nitrate reductase complex may take place in a unidirectional way.

This publication has 2 references indexed in Scilit: