Size fractionation of DNA fragments by liquid-liquid chromatography
Open Access
- 20 December 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 7 (8) , 2483-2499
- https://doi.org/10.1093/nar/7.8.2483
Abstract
A method for the fractionation of double-stranded DNA fragments from 150 to 22000 b.p. in size by liquid-liquid chromatography is described. The procedure makes use of the fact that the partitioning of DNA in a polyethylene glycol-dextran system is size dependent and can be altered by alkali metal cations. Cellulose or celite are used as supports for the stationary, dextran-r1ch phase. Examples show the fractionation of digests of T7 DNA produced by Dpn II and H1nd II restriction endonucleases as well as λ DNA digests produced by Hind III and Eco RI restriction endonucleases.Keywords
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