pH Gradient Layers for the TLC of Fluorescent Dyes

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Abstract
The advantages of pH gradient layers are presented with some fluorescent dyes as examples. In chromatography, transverse to the gradient (T-gradient) indications as to the chemical structure of the substance are obtained and contaminants are easily recognized. Chromatography in the direction of the gradient leads to a focusing, especially of the accompanying substances in the upper region of the chroma togram: These layers are suitable also for the chromatography of mixtures of substances of strongly differing polarities.