Cryo-electron microscopy of viruses
- 1 March 1984
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 308 (5954) , 32-36
- https://doi.org/10.1038/308032a0
Abstract
Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be prepared for observation by cryo-EM in easily controlled conditions. The viral particles appear free from the kind of damage caused by dehydration, freezing or adsorption to a support that is encountered in preparing biological samples for conventional EM. Cryo-EM of vitrified specimens offers possibilities for high resolution observations that compare favorably with any other EM method. [Animal viruses and bacteriophages were tested in this study.].Keywords
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